今日药学

2001, (06) 18-20

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动态比浊法鲎试验定量测定参麦注射液的细菌内毒素含量
Application of the kinetic turbidimetric limulus test to Shen Mai injection

劳海燕,罗宇芬,林秋晓
Lao Haiyan,Luo Yufen,Lin Qiuxiao(Pharmaceutical Department of Guangdong Provincial People's Hospital, Guangzhou 510080)

摘要(Abstract):

目的 :应用动态比浊法鲎试验定量测定参麦注射液的细菌内毒素含量。方法 :通过对 3批样品中定量添加标准内毒素的干扰试验 ,其回收率分别为 90 78%、80 0 3%、79 2 2 %。结果 :将样品进行 1/ 30稀释 ,样品中定量添加标准内毒素 ,回收率均在 5 0 %~ 2 0 0 % ,无干扰作用。结论 :动态比浊法鲎试验可以高效地测定参麦注射液的细菌内毒素
Objective:The kinetic turbidimetric limulus test was used in the endotoxin assay of Shen Mai injection. Methods:The validation test was fulfiled,when the amount of endotoxin spiked in Shen Mai injection sample were recovered in 90.78%,80.03%,79 22%.Results:The 30 fold dilution to Shen Mai injection is effective to eliminate the interference in limulus test. Conclution:The kinetic turbidimetric limulus test provide a new way to the detection of endotoxin in Shen Mai injection.

关键词(KeyWords): 细菌内毒素;动态比浊法鲎试验;参麦注射液;干扰试验
Bacterial endotoxin;Kinetic turbidimetric limulus test; Shen Mai injection; Inhibition enhancement test

Abstract:

Keywords:

基金项目(Foundation): 广东省重点科技攻关项目 ;项目编号 :2KMO5 40 6 3

作者(Author): 劳海燕,罗宇芬,林秋晓
Lao Haiyan,Luo Yufen,Lin Qiuxiao(Pharmaceutical Department of Guangdong Provincial People's Hospital, Guangzhou 510080)

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