今日药学

2019, v.29(03) 166-169

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4种广东常用清热解毒类中草药抑菌作用的研究
Study on Antibacterial Activities of 4 Chinese Herbal Medicine Commonly Used in Guangdong

萧栋;陈瑜珍;莫小路;
XIAO Dong;CHEN Yuzhen;MO Xiaolu;Guangdong Pharmaceutical University;Guangdong Food and Drug Vocational College;

摘要(Abstract):

目的研究鸡屎藤、布渣叶、火炭母、扭肚藤提取物的体外抑菌作用,为天然抗菌药物的开发提供试验依据。方法采用70%的乙醇水溶液分别对4种中草药进行超声波提取,以金黄色葡萄球菌、大肠杆菌和绿脓杆菌为供试细菌,利用K-B纸片琼脂扩散法对4种中草药的提取液进行体外抑菌试验,测定其抑菌活性,并测定其最小抑菌浓度(MIC)。结果 4种中草药对金黄色葡萄球菌和绿脓杆菌都有不同程度的抑制作用,但对大肠杆菌几乎无抑制作用;其中,嫩鸡屎藤、布渣叶、火炭母和扭肚藤对金黄色葡萄球菌有较强的抑制作用,MIC均为0.625 g·m L-1;而火炭母和扭肚藤对绿脓杆菌的抑制作用最强,MIC均为0.625 g·m L-1。结论 4种供试中草药都有不同程度的抑菌作用。
OBJECTIVE To investigate the antibacterial activities of Paederia scandens( Lour.) Merr,Microcos Paniculata L,Polygonum Chinense Linn and Jasminum elongatum( Bergius) in vitro. METHODS 4 herbs were extracted with ethanol by ultrasonic method respectively. Staphylococcus aureus,Escherichia coli and pseudomonas aeruginosa were used as the test bacteria,and Kirby-Bauer disc agar diffusion method was used to test the antibacterial activity of 4 Chinese herbs in vitro. Finally,the minimum inhibitory concentration( MIC) of the herb extract was determined by twice-fold dilution method. RESULTS All of the 4 tested Chinese herbs had inhibitory effect on Staphylococcus aureus and Pseudomonas aeruginosa,while no inhibitory effect on Escherichia coli,except for Jasminum elongatum( Bergius) Willd. Among the 4 tested herbs,the green part of Paederia scandens( Lour.) Merr,Microcos Paniculata L,Polygonum Chinense Linn and Jasminum elongatum( Bergius) Willd had strong inhibition on the Staphylococcus aureus.while Pseudomonas aeruginosa was strongly inhabited by Polygonum Chinense Linn and Jasminum elongatum( Bergius) Willd.CONCLUSION The 4 tested Chinese herbal medicine have different antibacterial effects.

关键词(KeyWords): 中草药;抑菌作用;最小抑菌浓度
herbal medicine;antibacterial activity;minimum inhibitory concentration

Abstract:

Keywords:

基金项目(Foundation): 广东省省级科技计划项目(2015A040404029)

作者(Authors): 萧栋;陈瑜珍;莫小路;
XIAO Dong;CHEN Yuzhen;MO Xiaolu;Guangdong Pharmaceutical University;Guangdong Food and Drug Vocational College;

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