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2019, v.29(10) 684-686+690

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大孔树脂-HPLC法测定淡竹叶中7种黄酮化合物
Determination of Seven Flavonoids from Lophatherum Gracile Brongn by Macroporous Resin and HPLC

李博;聂阳;朱俊访;
LI Bo;NIE Yang;ZHU Junfang;Guangdong Food and Drug Vocational College;

摘要(Abstract):

目的建立大孔树脂-HPLC法同时测定淡竹叶中7种黄酮化合物(芦丁、槲皮素、荭草苷、异荭草苷、牡荆素、异牡荆素和木犀草素)的方法。方法采用D101型大孔树脂富集淡竹叶总黄酮;采用Agilent C_(18)色谱柱(250 mm×4.6 mm,5μm);以甲醇和0.5%醋酸水溶液为流动相,进行梯度洗脱;柱温25℃;流速1.0 m L·min(-1);检测波长为360 nm。结果芦丁在15.20~45.60μg·m L(-1);检测波长为360 nm。结果芦丁在15.20~45.60μg·m L(-1),槲皮素在16.32~48.96μg·m L(-1),槲皮素在16.32~48.96μg·m L(-1),荭草苷在15.04~45.12μg·m L(-1),荭草苷在15.04~45.12μg·m L(-1),异荭草苷在17.44~52.32μg·m L(-1),异荭草苷在17.44~52.32μg·m L(-1),牡荆素在14.88~44.64μg·m L(-1),牡荆素在14.88~44.64μg·m L(-1),异牡荆素在16.64~49.92μg·m L(-1),异牡荆素在16.64~49.92μg·m L(-1),木犀草素在15.20~45.60μg·m L(-1),木犀草素在15.20~45.60μg·m L(-1)范围内线性关系良好,平均回收率分别为97.3%、98.1%、96.8%、101.1%、102.1%、99.1%和103.7%。结论本方法同时测定了淡竹叶中的7种黄酮化合物,方法准确、重现性好。
OBJECTIVE To establish the method for the determination of seven flavonoids( rutin, quercetin, orientin,isoorientin,vitexin,isovitexin and luteolin) from Lophatherum gracile Brongn by Macroporous resin and HPLC. METHODS D101 Macroporous resin was used to enrich the total flavonoids in Lophatherum gracile Brongn. A HPLC method was established on Agilent C_(18) column with the mobile phase consisting of methanol and 0. 5% acetic acid. The flow rate was 1. 0 m L·min(-1)范围内线性关系良好,平均回收率分别为97.3%、98.1%、96.8%、101.1%、102.1%、99.1%和103.7%。结论本方法同时测定了淡竹叶中的7种黄酮化合物,方法准确、重现性好。
OBJECTIVE To establish the method for the determination of seven flavonoids( rutin, quercetin, orientin,isoorientin,vitexin,isovitexin and luteolin) from Lophatherum gracile Brongn by Macroporous resin and HPLC. METHODS D101 Macroporous resin was used to enrich the total flavonoids in Lophatherum gracile Brongn. A HPLC method was established on Agilent C_(18) column with the mobile phase consisting of methanol and 0. 5% acetic acid. The flow rate was 1. 0 m L·min(-1) with the column temperature at 25 ℃,and detection wavelength was 360 nm. RESULTS The calibration curves showed good linearity in the range of15.20-45.60 μg·m L(-1) with the column temperature at 25 ℃,and detection wavelength was 360 nm. RESULTS The calibration curves showed good linearity in the range of15.20-45.60 μg·m L(-1) of rutin,16. 32-48. 96 μg·m L(-1) of rutin,16. 32-48. 96 μg·m L(-1) of quercetin,15. 04-45. 12 μg·m L(-1) of quercetin,15. 04-45. 12 μg·m L(-1) of orientin,17. 44-52. 32 μg·m L(-1) of orientin,17. 44-52. 32 μg·m L(-1) of isoorientin,14. 88-44. 64 μg·m L(-1) of isoorientin,14. 88-44. 64 μg·m L(-1) of vitexin,16. 64-49. 92 μg·m L(-1) of vitexin,16. 64-49. 92 μg·m L(-1) of isovitexin and 15. 20-45. 60 μg·m L(-1) of isovitexin and 15. 20-45. 60 μg·m L(-1) of luteolin. And the average recoveries for rutin,quercetin,orientin,isoorientin,vitexin,isovitexin and luteolin were 97.3%,98.1%,96.8%,101.1%,102.1%,99.1% and 103.7%,respectively. CONCLUSION The method used to detect the seven flavonoids in Lophatherum gracile Brongn with accuracy and repeatability.

关键词(KeyWords): 淡竹叶;黄酮;大孔树脂;高效液相色谱
Lophatherum gracile Brongn;flavonoids;macroporous resin;HPLC

Abstract:

Keywords:

基金项目(Foundation): 广东食品药品职业学院自然科学研究项目(2017ZR027)

作者(Authors): 李博;聂阳;朱俊访;
LI Bo;NIE Yang;ZHU Junfang;Guangdong Food and Drug Vocational College;

参考文献(References):

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