杨志荟;喻樊;缪怡烨;沈净燕;
YANG Zhihui;YU Fan;MIU Yiye;SHEN Jingyan;Deparatment of Pharmacy,Yancheng Normal University;Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection;Nanjing University of Technology;

• 1:盐城师范学院药学院
• 2:江苏省滩涂生物资源与环境保护重点建设实验室
• 3:南京工业大学

摘要(Abstract)：

目的观察米诺地尔脂质体和醇质体对小鼠绒毛生长的影响,为米诺地尔醇质体和脂质体的临床研究提供参考数据。方法本文主要考察小鼠毛长、毛重、毛囊数量、真皮层厚度及角质层结构五个参考指标,精确测量毛长与毛重,取小鼠皮肤组织切片并进行伊红法染色,观察其真皮层厚度是否存在差异,记录毛囊数,采用傅里叶衰减全反射红外光谱(ATR-FTIR)技术,讨论不同剂型对小鼠角质层结构的影响。结果米诺地尔脂质体组小鼠最早长出毛发,其次为米诺地尔醇质体组小鼠、米诺地尔酊剂组小鼠,最后为阴性对照组小鼠,并且米诺地尔脂质体组小鼠的新生毛发的长度及最终毛重、毛囊数量和真皮层厚度都高于其他组,存在显著性差异。结论米诺地尔脂质体促毛发生长作用最为明显,米诺地尔酊剂与米诺地尔醇质体促毛发生长作用差异不明显。
OBJECTIVE The effect of minoxidil liposomes and minoxidil ethosomes on hair growth of mice are observed and provided the reference data for the clinical study. METHODS Five indexes,of length of hair,weight of hair,the number of hair follicles,dermal thickness and structure of horny layer were selected to evaluate the effect. Length and weight of hair were measured accurately. The difference of the thickness of mice dermis was observed,the number of hair follicles was recorded after taking the mice skin biopsy-and staining in eosin. ATR-FTIR was used to reflect the effect of different dosage forms on mice's horny layer. RESULTS The hair of mice in the minoxidil liposomal group grow earliest,and the group of minoxidil ethosomes,the group of minoxidil tincture,the group of the negative control are followed. In addition,the new hair length,the final gross weight,the number of hair follicle and thickness dermis of the mice in the minoxidil liposomes group were higher than other groups,and there were significant differences.CONCLUSION Minoxidil liposomes have the most prominent effect on promoting hair growth,minoxidil tincture and minoxidil alcohol plasmid not have obvious effect.

关键词(KeyWords)： 米诺地尔;脂质体;醇质体;绒毛生长
minoxidil;liposome;ethosome;hair growth

Abstract:

Keywords:

基金项目(Foundation): 江苏省自然科学基金面上项目(BK20181478)

作者(Author): 杨志荟;喻樊;缪怡烨;沈净燕;
YANG Zhihui;YU Fan;MIU Yiye;SHEN Jingyan;Deparatment of Pharmacy,Yancheng Normal University;Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection;Nanjing University of Technology;

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DOI:

参考文献(References)：

• [1]陈声利，孙建方．米诺地尔治疗脱发的药理和临床研究[J]．国外医学皮肤性病学分册，2004,30(5):276-278.
• [2]Aljuffali I A,Pan T L,Sung C T,et al. Anti-PDGF receptorβantibody-conjugated squarticles loaded with minoxidil for alopecia treatment by targeting hair follicles and dermal papilla cells[J]. Nanomedicine:nanotechnology,biology,and medicine,2015,11(6):1321-1330.
• [3]陈元岭，王丹，王技，等．茶多酚醇质体的制备和体外透皮吸收研究[J]．中国新药杂志，2013,22(15):1757-1778.
• [4]刘晓谦，王锦玉，仝燕，等．脂质体制备技术及其研究进展[J]．中国药学杂志，2011,46(14):1084-1088.
• [5]李晓锋．多囊系统在药物递送中的研究进展[J]．中国新药杂志，2019,28(14):1704-1712.
• [6]代秀菊，涂平，秦俭．米诺地尔对培养人头皮毛囊生长的影响[J]．临床皮肤杂志，2002,31(1):6-8.
• [7]汤杰，肖丽娟，罗琼，等．米诺地尔醇质体的制备[J]．中南药学，2009,7(7):510-513.
• [8]徐贤柱，魏允，饶华，等．绿原酸纳米脂质体制备与抑菌性分析[J]．食品科学，2014,35(20):62-66.
• [9]朱俊．米诺地尔溶液的制备及其质量的控制[J]．药学与临床研究，2010,18(5):425-426..
• [10]何红梅，朱红霞，刘强，等．何首乌提取物对C57BL/6J小鼠毛囊生长和毛发周期生长的影响[J]．中国实验方剂学杂志，2012,18(23):216-219
• [11]李丰文，朱兴祥，林碧荣，等．米诺地尔溶液的经皮吸收研究[N]．中国药科大学学报，2001,32(4):257-259.
• [12] Shen Y,Zhang Y,Liu N. Let-7b promotes alpaca hair growth via transcriptional repression of TGFβRI[J]. Gene,2016,577(4):32-36.
• [13]杨亚军，王冬春，周秋贵，等．水溶性米诺地尔对C57BL/6小鼠毛发生长影响的研究[J]．海峡药学，2007,19(7):34-36.
• [14] Jo S J,Choi S J,Yoon S Y. Valproic acid promotes human hair growth in in vitro culture model[J]. Journal of Dermatological Science,2013,72(12):16-24.
• [15]陈康，何文，张先洲．N-三甲基壳聚糖对青藤碱脂质体体外性质的影响[J]．广东药学院学报，2013,29(3):229-232.
• [16]潘燕飞．傅立叶红外光谱法用于茶叶品质的鉴定[J]．烟台大学学报(自然科学与工程版)，2008,21(4):266-272.
• [17]程凤仪，张振海，周建平，等．N-精氨酸壳聚糖对药物透皮促渗作用及其机制的探讨[J]．药学学报，2013,48(8):1325-1332.
• [18]韩向晖，李经才．脱发发病机理与防治药物新进展[J]．沈阳药科大学学报，2001,18(3):223-227.
• [19]周雪琴，许晶，赵晓东，等．氮酮对鼠角质层角蛋白作用的光谱证据[J]．高等学校化学学报，2004,25(7):1273-1276.
• [20]刘维，欧剑．米诺地尔纳米结构脂质载体的制备及配方优化[J]．武汉大学学报(医学版)，2012,33(2):202-205.
• [21]傅琳玲，朱文元，范卫新．米诺地尔对培养鼠触须毛囊生长的影响[J]．中华皮肤科杂志，1998,31(6):371-372.