今日药学

2014, v.24(05) 331-333

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IL-27对慢性丙肝患者外周血Th1/Th17水平调节作用的研究
Effect of IL-27 on Level of Th1 / Th17 in Peripheral Blood of Patients with Chronic Hepatitis C

余晓霞;伍俊妍;丁亮;李国成;
YU Xiao-xia;WU Jun-yan;DING Liang;LI Guo-cheng;Department of Pharmacy,the Second Affiliated Hospital of Sun Yat-set University;

摘要(Abstract):

目的探讨IL-27体外对慢性丙肝患者外周血Th1/Th17水平调节作用。方法分离慢性丙型肝炎(Chronic Hepatitis C,CHC)患者和健康捐献者(Health Donor,HD)外周血单个核细胞(Peripheral Dlood Mononuclear Cells,PBMC),将IL-27加入CHC患者及HD的PBMC并培养72 h,CHC患者和HD外周PBMC组不做为空白对照,应用流式细胞仪检测IL-27刺激前后不同组PBMC中CD4+T细胞内IFN-γ和IL-17的变化情况,计算Th1、Th17细胞比例,观察体外IL-27对CHC患者和HD外周血Th1/Th17的影响。结果 IL-27刺激48 h后Th1细胞亚群变化不明显,Th17细胞比例明显下降(P=0.01)。72 h后CHC+IL-27组Th1细胞比例较CHC组升高(P=0.04),Th17细胞比例仍表现为下降趋势(P=0.02),变化较HD组更加明显。结论 IL-27可以刺激慢性丙肝患者Th1/Th17的变化,表现为IL-27提高患者免疫系统中Th1细胞的比例,降低Th17细胞比例。
Objective To evaluate the effect of IL-27 on the level of Th1 /Th17 in patients with chronic hepatitis C(CHC) in vitro.Methods The peripheral blood mononuclear cells(Peripheral Dlood Mononuclear Cells,PBMC) of patients with CHC and health donor(HD) were isolated.IL-27 was added into PBMC and cultured it in vitro with PBMC alone as control.Flow cytometry was used to detect the IFN-γ and IL-17 changes in CD4 + T cell of PBMC in each group.Then the ratio of Th1,Th2 cells were calculated,and the effect of IL-27 on Th1 / Th17 in patients CHC in vitro was assessed.Results By stimulating for 48 h,IL-27 could inhibit proliferation of lymphocyte in vitro.Compared to baseline,CHC + IL-27 group did not showed a rising trend in Th1 cell,while Th17 cells present down trend(P = 0.01).After 72 h,CHC + IL-27 group showed a rising trend in Th1 cell(P = 0.04) and Th17 cells still present down trend(P = 0.02) compared with that of the control group and HD group.Conclusion IL-27 played a regulatory role in Th1 / Th17 in patients with CHC in vitro,by rising the ratio of Th1 cells and decreasing the Th17 cells.

关键词(KeyWords): IL-27;慢性丙型肝炎;Th1/Th17
IL-27;chronic hepatitis C;Th1 /Th17

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基金项目(Foundation): 广东省医院药学研究基金(编号:2013A06)

作者(Author): 余晓霞;伍俊妍;丁亮;李国成;
YU Xiao-xia;WU Jun-yan;DING Liang;LI Guo-cheng;Department of Pharmacy,the Second Affiliated Hospital of Sun Yat-set University;

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