今日药学

2020, v.30(08) 541-543

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

ACYW135群脑膜炎球菌多糖疫苗中乳糖含量测定
Content Determination of Lactose in ACYW135 Meningococcal Polysaccharide Vaccine

周志平;韩斌;董洁;李阳;李世光;
ZHOU Zhiping;HAN Bin;DONG Jie;LI Yang;LI Shiguang;Hua Lan Biological Bacterin Co.Ltd;

摘要(Abstract):

目的建立高效液相色谱法测定乳糖含量的方法。方法研究了流动相、流速及柱温等因素对试验结果的影响,确定了乳糖检测方法的最佳试验条件:色谱柱为AminexHPX-87H柱(300 mm×7.8 mm,17μm),流动相为乙腈-水(60∶40,V/V),流速1.0 mL·mL~(-1),柱温为45℃,进样量10μL。结果试验表明,乳糖浓度在1.25~20.00 mg·mL~(-1)范围内与色谱峰面积呈线性关系,相关系数为r=1,方法检出限为0.02 mg·mL~(-1),定量限为0.062 5 mg·mL~(-1),相对标准偏差(RSD)为0.78%(n=9)。结论该方法操作简便、准确度高、特异性较好,可用于ACYW135群脑膜炎球菌多糖疫苗中乳糖含量测定。
OBJECTIVE To Determine the content of lactose by High Performance Liquid Chromatography method. METHODS A series of experiment parameters such as mobile phase,flow rate and column temperature were optimized and the optimal conditions were chosen: AminexHPX-87H(300 mm× 7.8 mm,17 μm) column was used,acetonitrile-water(60 ∶ 40,V/V) as mobile phase,1.0 mL·mL~(-1) as flow rate,45 ℃ as column temperature and 10 μL as sample volume. RESULTS The results show that the concentration of lactose was within the range of 1. 25-20. 00 mg·mL~(-1) and linearly dependent on the peak's area. The relative coefficient was 1. The detection limit was 0.02 mg·mL~(-1) and quantitative limit was 0.062 5 mg·mL~(-1). The relative standard deviation(RSD) was 0.78%(n = 9). CONCLUSION This method is easy to operate and has good accuracy and selectivity. Thus,this analytical method is suitable for the content determination of lactose in ACYW135 meningococcal polysaccharide vaccine.

关键词(KeyWords): 高效液相色谱法;多糖疫苗;乳糖;含量测定
HPLC;meningococcal vaccine;lactose;content determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 周志平;韩斌;董洁;李阳;李世光;
ZHOU Zhiping;HAN Bin;DONG Jie;LI Yang;LI Shiguang;Hua Lan Biological Bacterin Co.Ltd;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享