今日药学

2009, v.19(11) 53-55

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高效液相色谱法测定盐酸麻黄碱滴鼻液的含量
Determination of Ephedrine Hydrochloride in Ephedrine Hydrochloride Nasal Drops by HPLC

周日笑;肖晓兰;
ZHOU Ri-xiao,XIAO Xiao-lan (Guangdong Yangjiang Institute for Drug Control,Yangjiang,Guangdong 529500,China)

摘要(Abstract):

目的建立用高效液相色谱(HPLC)法测定盐酸麻黄碱滴鼻液中盐酸麻黄碱含量的方法。方法选用Diamonsil(钻石)C18(5μm,150 mm×4.6 mm)为分析柱;检测波长为215 nm;柱温为35℃;甲醇-0.01 mol/L磷酸二氢钾(20∶80)为流动相;流速为0.8 ml/min。结果盐酸麻黄碱的保留时间约为7.9 min,在43.776~136.800μg/ml的浓度范围内线性关系良好(r=0.9999)。平均回收率为100.4%,相对标准偏差值(RSD)为0.59%;供试品溶液在18 h内稳定。结论该方法精密度好,准确度高,简单易行。
Objective To develop a method for determination of Ephedrine Hydrochloride in Ephedrine Hydrochloride nasal drops by HPLC.Methods The HPLC system was consisted of a Diamonsil(diamond) C 18(5 μm,150 mm×4.6 mm) at 35℃,and the UV-vis detective wave length was 215 nm.The mobile phase was composed of Methanol-0.01 mol/L Potassium Dihydrogen Phosphate(20∶80),and the flowing rate was 0.8 ml/min.Results The retention time of Ephedrine Hydrochloride was about 7.9 min.Ephedrine Hydrochloride had good linearity within the range of 43.776~136.800 μg/ml(r= 0.9999).The average recovery was 100.4%,and RSD was 0.59%.Sample solution was stable within 18 h.Conclusion The developed method is simple,fast,and sensitive,thus suitable for the analysis of Ephedrine Hydrochloride in Ephedrine Hydrochloride nasal drops.

关键词(KeyWords): 高效液相色谱法;盐酸麻黄碱;盐酸麻黄碱滴鼻液
HPLC;Ephedrine Hydrochloride;Ephedrine Hydrochloride nasal drops

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作者(Author): 周日笑;肖晓兰;
ZHOU Ri-xiao,XIAO Xiao-lan (Guangdong Yangjiang Institute for Drug Control,Yangjiang,Guangdong 529500,China)

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