叶小英;谢煜敏;曾聪彦;
YE Xiaoying;XIE Yumin;ZENG Congyan;Zhongshan South China Chinese Medicine Park Development Co.,Ltd.;Research Centre of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine,Key Laboratory of Chinese Medicinal Resource from Lingnan,Ministry of Education;Zhongshan Hospital of Traditional Chinese Medicine;

• 1:中山市华南现代中医药城发展有限公司
• 2:广州中医药大学中药资源科学与工程研究中心岭南中药资源教育部重点实验室
• 3:中山市中医院

摘要(Abstract)：

番石榴多糖是番石榴的最主要化学成分和有效成分之一，具有降血糖、抗氧化、抗糖化、抗菌、免疫调节等生物活性。本综述主要概述了番石榴多糖的化学成分，提取、分离与纯化以及番石榴多糖的药理作用等方面内容，以期为番石榴多糖的深入研究与开发利用提供参考。
The Psidium guajava L.polysaccharide,which is one of the most important chemical and effective constituents of guava,has various biological activities,such as hypoglycemic,antioxidant,anti-glycated,antibacterial,regulating immune effects.To provide reference for further research and development of Psidium guajava L.polysaccharide,chemical constituent,extraction,separation and purification,pharmacological action of Psidium guajava L.polysaccharide were summarized in this review.

关键词(KeyWords)： 番石榴;多糖;化学成分;提取分离纯化;药理作用
Psidium guajava L;polysaccharide;chemical constituent;extraction,separation and purification;pharmacological action

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 叶小英;谢煜敏;曾聪彦;
YE Xiaoying;XIE Yumin;ZENG Congyan;Zhongshan South China Chinese Medicine Park Development Co.,Ltd.;Research Centre of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine,Key Laboratory of Chinese Medicinal Resource from Lingnan,Ministry of Education;Zhongshan Hospital of Traditional Chinese Medicine;

参考文献(References)：

• [1]刘晓聪,林冬梅,刘敏,等.番石榴的化学成分及其抗肿瘤与抗真菌活性[J].中国中药杂志,2021,46(15):3877-3885.
• [2]Pasupuleti V,Kulkarni S G.Lycopene fortification on the quality characteristics of beverage formulations developed from pink flesh guava(Psidium guajava L.)[J].Journal of Food Science and Technology,2014,51(12):4126-4131.
• [3]朱韵辰,林星.甘草多糖药理学作用研究进展[J].中国现代应用药学,2021,38(21):2763-2768.
• [4]华德洪.番石榴多糖的分离纯化、结构鉴定及生物活性测试[D].广州:广东药学院,2014.
• [5]Khawas S,SivováV,Anand N,et al.Chemical profile of a polysaccharide from Psidium guajava leaves and it s in vivo antitussive activity[J].International Journal of Biological Macromolecules,2018,109(1):681-686.
• [6]Lin H C,Lin J Y.Characterization of guava(Psidium guajava Linn)seed polysaccharides with an immunomodulatory activity[J].Int JBiol Macromol,2020,154(5):11-20.
• [7]罗游.番石榴叶多糖活性分析及分离鉴定与发酵提升[D].广州:华南理工大学,2020.
• [8]Wang X,Lu X.Characterization of pectic polysaccharides extracted from apple pomace by hot-compressed water[J].Carbohydrate Polymers,2014.102(15):174-184.
• [9]扎罗,刘振东,王波,等,复合酶法提取西藏金耳粗多糖工艺研究[J].轻工科技,2018,34(1):23.
• [10]Ogutu F O.Ultrasonic Modification of Selected Polysaccharides-Review[J].Journal of Food Processing&Technology, 2015,6(5):1-8.
• [11]邱敏芳,黎莉,方继德,等.番石榴多糖提取工艺的优化[J].武汉工程大学学报,2011,33(1):25-27.
• [12]Mandal S.,Sarlcar R,Patra P,et al.Structural studies of a heteropolysaccharide(PS-1)isolated from hot water extract of fruits of Psidium guajava(Guava)[J].Carbohydrate Research,2009,344(11):1365-1370.
• [13]蒋顶云,蒋长兴,熊清平,等.响应面法优化番石榴多糖的超声提取工艺[J].食品科技,2012,37(12):174-178.
• [14]孔繁晟,明建斌,黄志鹏,等,响应面分析法优选番石榴果实中多糖的超声提取工艺[J].中国药房,2014,25(15):1382-1385.
• [15]Amutha G,Arasi M A,Gopal R M,et al.Optimization of microwaveassisted extraction of polysaccharide from Psidium guajava L.fruits[J].Int J Biol Macromol,2016,91:227-232.
• [16]李珊,梁俭,冯彬,等,番石榴多糖的提取工艺优化、纯化及其抗氧化活性测试[J].粮食与油脂,2020,33(7):68-73.
• [17]杜阳吉,王三永,李春荣.番石榴叶中多糖分离纯化工艺研究[J].中国食品添加剂,2011,32(1):156-159.
• [18]Luo Y,Peng B,Liu Y,et al. Ultrasound extraction of polysaccharides from guava leaves and their antioxidant and antiglycation activity[J].Process Bionhemistry,2018,73:228-234.
• [19]Kong F,Yu S,Zeng F,et al.Optimization of ultrasonic-assisted extraction of antioxidant compounds from Guava(Psidium guajavaL.)leaves using response surface methodology[J].Pharmacogn Mag,2015,11:463.
• [20]Das A,Majumder K.Fractional changes of pectic polysaccharides in different tissue zones of developing guava(Psidium guajava L.)fruits[J].Scientia Horticulturae,2010,125(3):406-410.
• [21]Soares F D,Pereira T,Marques M O M,et al.Volatile and non-volatile chemical composition of the white guava fruit(Psidium guajava)at different stages of maturity[J].Food Chemistry,2007,100(1):15-21.
• [22]侯峰.番石榴叶和幼果降糖成分开发研究[D].广州:华南理工大学,2011.
• [23]Lee J H,Park H M,Nam D,et al.Mixture of guava leaves extract and Citrus hassaku pericarp extract inhibits in vitro inflammatory biomarkers by blocking ERK,JNK,and p38 MAPK signaling and protects mice from lethal endotoxemia[J].Oriental Pharmacy and Experimental Medicine,2015,15(1):13-19.
• [24]Sriwilaijaroen N,Fukumoto S,Kumagai K,et al.Antiviral effects ofPsidium guajava Linn.(guava)tea on the growth of clinical isolated H1N1 viruses:Its role in viral hemagglutination and neuraminidase inhibition[J].Antiviral Research,2012,94(2):139-146.
• [25]Yan C Y,Lee J S,Kong F S,et al.Anti-glycated activity prediction of polysaccharides from two guava fruits using artificial neural networks[J].Carbohydrate Polymers,2013,98(1):116-121.
• [26]Jiao Y K,Zhang M L,Wang S M,et al.Consumption of guava may have beneficial effects in type 2 diabetes:A bioactive perspective[J].International Journal of Biological Macromolecules,2017, 101:543-552.
• [27]Nair S S,Vaibhavi K,Anshu M.In.vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plantextracts[J].European Journal of Experimental Biology,2013(3):128-132.
• [28]焦峪坤.两种植物多糖的分离纯化及活性研究[D].广州:广东药科大学,2017.
• [29]Zhang Z L,Kong F S,Ni H,et al.Structural characterization,α-glucosidase inhibitory and DPPH[rad]scavenging activities of polysaccharides from guava[J]. Carbohydrate Polymers,2016,144:106-114.
• [30]王占一,孔德营,戴博,等.石榴叶多糖亚临界水提取工艺的优化及其外抗氧化活性[J].中成药,2017,39(10):2039-2044.
• [31]吴建中,欧仕益,陈静,等.番石榴多糖对糖尿病小鼠的血糖及抗氧化能力的影响[J].中成药,2007(5):668-671.
• [32]黄艳,周璐丽,王定发,等.番石榴叶提取物体外抑菌和抗氧化作用的研究[J].黑龙江畜牧兽医,2017(21):192-194,199.
• [33]赵天野.番石榴果、叶水提液体外抑菌效果的比较研究[J].生物化工,2020,6(4):83-85.
• [34]陈淳,林清洪,林志楷,等.番石榴叶黄酮类化合物提取及其粗提物对蔬菜病原真菌的抑菌活性[J].亚热带植物科学,20 15,44(4):284-288.
• [35]Wang L,Xie J,Huang T,et al.Characterization of silver nanoparticles biosynthesized using crude polysaccharides of Psidium guajava L.leaf and their bioactivities[J].Materials Letters,2017,208:126-129.
• [36]Hoseinifar S H,Sohrabi A,Paknejad H,et al.Enrichment of common carp(Cyprinus carpio)fingerlings diet with Psidium guajava:The effects on cutaneous mucosal and serum immune parameters and immune related genes expression[J].Fish and Shellfish Immunology,2019,686:688.
• [37]Lin H C,Lin J Y.Immune cell-conditioned media suppress prostate cancer PC-3 cell growth correlating with decreased proinflammatory/anti-inflammatory cytokine ratios in the media using 5 selected crude polysaccharides[J].Integrative Cancer Therapies,2016,15(4):13-25.
• [38]Lin H C,Lin J Y.Splenocyte-conditioned media inhibit breast cancer MCF-7 cell growth,associated with increased Th2/Th1 cytokine secretion ratio in the media using five selected polysaccharides[J].Journal of Immunobiology,2017,2(2):1-14.
• [39]Bartolome A,Mandap K,David K,et al.SOS-red fluorescent protein(RFP)bioassay system for monitoring of antigenotoxic activity in plant extracts[J].Biosensors&Bioelectronics,2006,21(11):2114-2120.
• [40]Amith K,Reshma K,Rajalakshmi R,et al.Radiomodulatory Role of Psidium guajava Leaf Extracts against X-ray Induced Genotoxicity,Oxidative stress and Apoptosis in albino Wistar Rat Model[J].Journal of Applied Pharmaceutical Science,2016,6(3):58-65.
• [41]Lin H,Chien L,Jin Y.GSF3, a polysaccharide from guava(Psidium guajava L.)seeds,inhibits MCF-7 breast cancer cell growth via increasing Bax/Bcl-2 ratio or Fas mRNA expression levels.[J].International Journal of Biological Macromolecules,2020,161(15):1261-1271.
• [42]Lin H C,Lin J Y.Pharmacological Effects of Guava(Psidium guajava L.)Seed Polysaccharides:GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro[J].Molecular,2021(7):3631-3639.
• [43]Luo Y,Peng B,Wei W,et al.Antioxidant and Anti-Diabetic Activities of Polysaccharides from Guava Leaves[J].Molecules,2019,24(7):1343.
• [44]Aspalilan A,Faizah O,Abdul R,et al.Supplementation of Psidium guajava(Guava)Fruit Polysaccharide Attenuates Paracetamol-induced Liver Injury by Enhancing the Endogenous[J].Antioxidant.Activity Sains Malaysiana,2015(8):1129-1136.
• [45]潘汇,蔡为荣,谢亮亮,等.运用HSCCC法分离纯化荷叶中三种黄酮及抗氧化活性[J].食品工业科技,2018,39(16):161-165,170.
• [46]吕露阳,张志锋.HSCCC法分离纯化白花丹根中的兰雪醌[J].中国医药工业杂志,2017,48(6):850-853.
• [47]庄虎.石榴皮渣果胶乳化特性及其应用研究[D].武汉:湖北工业大学,2019.
• [48]Deguchi Y,Miyazaki K.Anti-hyperglycemic and anti-hyperlipidemic effects of guava leaf extract[J].Nutrition&Metabolism,2010,7(1):1-10.
• [49]上海第一医学院中山医院中医科消化科.番石榴片治疗糖尿病初步小结[J].广西医学院学报,1980,10(1):38-40.