徐群英,董淳,常翠
XUE Qunying;DONG Chun; CHANG Cui The People's Hospital of Shenzhen, The Second Affiliated Hospital of Jinan University. Shenzhen, Shenzhen 518020

• 1:深圳市人民医院
• 2:深圳市人民医院
• 3:深圳市人民医院 暨南大学第二附属医院
• 4:广东 深圳 518020

摘要(Abstract)：

目的 探讨不同剂量黄芪(47.62％、15.87％、5.29％)组方拯阳汤对小鼠免疫功能的影响。方法 建立受氢化可的松(HC)免疫抑制动物模型,采用MTT法检测腹腔巨噬细胞(Mφ)的活性、ConA诱导的脾脏T淋巴细胞转化增殖;以YAC-1为靶细胞用LDH释放改良法测定小鼠脾脏NK细胞和LAK细胞毒活性。结果 不同剂量黄芪组方的拯阳汤具有不同程度地拮抗HC对小鼠腹腔Mφ的活性的抑制,拮抗HC对小鼠脾脏T淋巴细胞转化的抑制,拮抗HC对小鼠脾脏NK细胞和LAK细胞毒活性的抑制。结论 拯阳汤具有拮抗HC对小鼠细胞免疫抑制的作用。
Objective To study the effect of Zheng Yang Tang with different doeses of Astragolus moholicus on the influence of Immu- nological Functions in the Immunosuppressive Mice by Hydrocortisone(HC). Methods Immunosuppressive animal model was estab- lished by HC. Examined the phagoctic function of peritoneal Mφ and the proliferation rate of T cells in spleen in response to induction of ConA by MTT chromometry. In addition, examined the cytotoxicity of NK cells and LAK cells in spleen agninst target cell, YAC- 1, by the method of LDH. Results with different doeses of Astragolus moholicus, zheng Yang Tang had different influence on the phagoctic function of peritoneal Mφ and on the proliferation rate of T cells in spleen in response to induction of ConA, also on the cyto- toxicity of NK cells and IAK cells,in the Immunosuppressive Mice by HC. Conclusion Zheng Yang Tang could evidently improve all the immnological functions in HC- induced immunosuppressive mice.

关键词(KeyWords)： 拯阳汤;黄芪;氢化可的松;免疫抑制;免疫功能
Zheng Yang Tang;hydrocortisone;immunosuppression;immunological function

Abstract:

Keywords:

基金项目(Foundation): 深圳市科技局专项(编号20030405)

作者(Authors): 徐群英,董淳,常翠
XUE Qunying;DONG Chun; CHANG Cui The People's Hospital of Shenzhen, The Second Affiliated Hospital of Jinan University. Shenzhen, Shenzhen 518020

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