今日药学

2001, (05) 9-11

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细菌内毒素定量测定法(动态比浊法)测定茵栀黄注射液中细菌内毒素含量
Application of the kinetic turbidimetric limulus test in the endotoxin assoy of Yin Zhi Huang injection

林秋晓,劳海燕,李智辉
Lin Qiuxiao,Lao Haiyan,Li Zhihui (Pharmaceutical Department of Guangdong Provincial People's Hospital,Guangzhou 510080)

摘要(Abstract):

目的 :应用细菌内毒素定量测定法 (动态比浊法 )定量测定茵栀黄注射液中的细菌内毒素含量。方法 :通过对样品中定量添加标准内毒素的干扰试验 ,检测其回收率应在 5 0 %~ 2 0 0 %范围。结果 :将样品进行1/ 30稀释可以有效地消除其对鲎试验的干扰。结论 :细菌内毒素定量测定法 (动态比浊法 )可以高效地测定样品中的细菌内毒素含量。
Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Yin Zhi Huang injection.Methods:The validation test was fulfiled.The amount of endotoxin spiked into Yin Zhi Huang injection sample was recovered in 50%~200% range.Results:A 30-fold dilution to Yin Zhi Huang injection is effective to eliminate the interference in limulus test.Conclusion:The kinetic turbidimetric Limulus test provide a new way for the detection of endotoxin in Yin Zhi Huang injection.

关键词(KeyWords): 细菌内毒素;动态比浊法;茵栀黄注射液;干扰试验
kinetic turbidimetric limulus test;Yin Zhi Huang injection;Inhibition enhancement test;bacterial endotoxin

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基金项目(Foundation): 广东省重点科技攻关项目 ;;项目编号 :2KM0 5 4 0 63

作者(Author): 林秋晓,劳海燕,李智辉
Lin Qiuxiao,Lao Haiyan,Li Zhihui (Pharmaceutical Department of Guangdong Provincial People's Hospital,Guangzhou 510080)

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